Expression and significance of miR-193b in cervical cancer

AIM: To investigate the expression of microRNA-193b(miR-193b) in the cervical tissues, and further to explore the effect of silencing miR-193b on diamminedichloroplatinum(DDP)-treated HeLa cell viability. METHODS: The expression levels of miR-193b in different cervical tissues were examined by qPCR. After transfection of miR-193b-inhibitor, the cell migration was determined by Transwell assay, the sensitivity of HeLa cells to DDP was measured by MTT assay, the protein levels of phosphate and tension homology deleted on chromsome ten(PTEN), protein kinase B(Akt), p-Akt and p-glycoprotein(P-gp) were determined by Western blot. RESULTS: The mRNA level of miR-193b was significantly increased in the cervical cancer tissues compared with normal cervical tissues(P<0.05). Knockdown of miR-193b obviously inhibited migration and enhanced sensitivity to DDP of HeLa cells(P<0.05). Additionally, after transfection of miR-193b-inhibitor, the expression of PTEN was increased, whereas the protein levels of p-Akt and P-gp were decreased(P<0.05). CONCLUSION: miR-193b is highly expressed in the cervical cancer tissues. Inhibition of miR-193b augments the sensitivity to DDP of HeLa cells, at least in part, through PTEN-PI3K/Akt signaling pathway.     

氮氧自由基化合物对L7712细胞DNA RNA和蛋白质合成的抑制作用

2,2,6,6—四甲基哌啶氮氧自由基等自由基化合物,均比其还原体化合物对癌细胞DNA合成的抑制率高。四甲基哌啶氮氧自由基和四甲基吡咯啉氮氧自由基对L7712细胞DNA,RNA和蛋白质合成的抑制作用,在3—24小时内均随作用时间延长而增高,它们对癌细胞DNA合成的抑制作用机理,似属干扰代谢型。     

肺癌腔内后装治疗方法改进的研究

对７０例肺癌患者随机分为两组，用改进麻醉及插管方法与常规方法作了对经研究，结果表明：在改进法组中，患者剧烈咳嗽，支气管痉挛和咯血等并发症明显减少，并对两组并发症的治疗作了讨论。     

ULTRASONOGRAPHIC FEATURES OF PARAPROSTATIC CYSTS IN NINE DOGS

The ultrasonographic features of paraprostatic cysts in nine dogs are described along with historical, clinical, surgical and pathologic findings. Cysts occurred predominantly in older, large breed dogs (mean age 8 years, range 3–11 years). The most common presenting complaints were depression, inappetance, stranguria, tenesmus and bloody penile discharge. A palpable abdominal mass was the most common physical finding. Ultrasonographically, paraprostatic cysts were usually large anechoic structures; many contained internal septa. Moderately large anechoic cavities or cysts were also detected in the prostatic parenchyma of five dogs, and in two of these communication with the paraprostatic cyst was visualized. There were no clearly distinct ultrasonographic criteria to discriminate septic from nonseptic paraprostatic cysts.     

PROSTATIC REFLUX DURING POSITIVE CONTRAST RETROGRADE URETHROGRAPHY IN THE DOG

Forty-five positive contrast retrograde urethrograms in which contrast medium reflux into the prostate was identified were evaluated to determine whether a correlation existed between the time and pattern of reflux and the nature of the prostatic disease. Prostatic diseases identified included prostatitis (25 dogs), prostatic tumor (13 dogs), and prostatic hyperplasia (1 dog). Six dogs with contrast medium reflux had no evidence of prostatic disease. No correlation was found between presence of prostatic reflux and specific prostatic diseases; however, prostatic tumor or infection was observed more frequently than hyperplasia, metaplasia, or cyst in the dogs with contrast medium reflux. A linear pattern and irregular, indistinct margination of the contrast medium reflux were associated with prostatitis; however, these patterns were also observed in dogs without prostatic disease     

TRANSABDOMINAL SONOGRAPHIC EVALUATION OF THE CANINE PROSTATE

The present study sought to determine the accuracy of ultrasonographic measurement of the prostate; to observe the ultrasonographic appearance of the normal prostate; and to compare these observations with those in prostates affected by cystic hyperplasia, benign hyperplasia, land neoplasia. Trassabdominal ultrasonography of the canine prostate was performed in eight normal dogs to establish criteria for evaluating enlarged prostates. Marginal boundries, size, and ultrasonographic architecture were compared with dissection findings to assess the accuracy of ultrasonography. Four enlarged prostates were evaluated ultrasonographically, and the findings were correlated with results of needle aspiration and urethral wash and with surgical and histopathologic findings. Differentiation between cystic and solid prostatic enlargement by ultrasonography proved efective. Transducer frequency and gain manipulation were important factors in describing the nature of the fluid within a cyst. Assenssment of the nature of solid prostatic enlargement and accurate size determination proved to be areas in which ultrasonography should by used in conjunction with other diagnostic techniques.     

Correlations between acute radiation scores and pain scores in canine radiation patients with cancer of the forelimb

Objective To determine whether there is a correlation between skin acute radiation score (ARS) and pain scores and to determine if skin ARSs can be used to predict future pain scores and increased need for analgesia in dogs undergoing radiation therapy for cancer of the forelimb. Study design Prospective observational study. Animals Seven middle‐aged dogs of various breeds with cancer of the forelimb. Methods Each neoplasm was surgically removed and a histologic diagnosis was obtained. Curative intent radiation therapy was initiated 2½–4½ weeks after surgery. Curative intent radiation therapy was delivered as prescribed. Two trained observers scored the dogs using a visual analog pain scale (VAS), Glasgow composite measure of pain scale, short form (GCMPS) and skin ARS prior to each day’s therapy. Daily scores were averaged and scatter plots were developed. Generalized estimating equation regressions were used to calculate standard error, 95% confidence interval, and p‐values for each relationship. Confidence and prediction bands were plotted. Results A statistically significant correlation between skin ARS and VAS and GCMPS pain scores was identified indicating that as the skin ARS increased so did the pain scores. A general correlation between VAS and GCMPS scores was observed. Early (fraction days 1–6) GCMPS scores were significantly influenced by anxiety behavior unrelated to pain. Skin ARS was found to predict precisely current and future presence of pain, but could only predict a range of potential future pain scores based on the pain management approach in use during this study. Conclusions Skin ARS can provide valuable information for initiating preemptive analgesia and intensifying pain management during curative intent radiation therapy. Daily pain scoring with an acceptable pain scale should be used in conjunction with the skin ARS to improve patient pain management. Clinical relevance Pain is an anticipated consequence of curative intent radiation therapy. Understanding the correlation between pain and skin ARS may facilitate more effective pain management.     

Correlation between the histopathological diagnosis by AgNOR count and AgNOR area in canine mammary tumors

Background: Mammary tumors are the most common type of tumor in female dogs. The histopathological diagnosis is usually made by a hematoxylin-eosin (HE) staining of the tumor, which then requires a pathologist's judgment for assessment of malignancy. The purpose of this study was to investigate an alternative silver staining of some argyrophilic nucleolar organizer regions (AgNOR) for improving the diagnostic accuracy with mammary tumors.
Hypothesis: There is a correlation between the histopathological diagnosis by AgNOR count and AgNOR area in canine mammary tumors.
Animals: Seventy-three canine mammary tumors from 33 female dogs.
Materials and Methods: The AgNOR staining was evaluated retrospectively in 73 canine mammary tumors with a parallel HE staining as a "Gold Standard." Both a quantitative manual counting method and a qualitative computerized morphometric method were tested.
Result: The result from both methods indicated a clinically relevant difference in the mean values of the AgNOR in the following 4 categories: malignant, benign, hyperplastic, and normal mammary tissue. The counting method was superior, with 89% of the cases given a correct diagnosis of a malignant or a nonmalignant canine mammary tumor. The 2 methods were then compared to test their ability to classify the tumors correctly. Again, the counting method was the most reliable method, with a sensitivity of 80% and a specificity of 76% when the upper 50% of the AgNOR counts were presumed malignant.
Conclusion and Clinical Importance: The results indicated that an AgNOR test could be an aid to pathologists as a prognostic indicator or to assist them in deciding between a benign or a malignant diagnosis in questionable cases.     

Breed differences in the proportional morbidity of testicular tumours and distribution of histopathologic types in a population-based canine cancer registry

Histologically verified tumours submitted to the Norwegian Canine Cancer Register from 1990 to 1998 were studied (n=14,401). The proportion of testicular tumours (n=345) was 2.4%, and the breakdown of histological tumour diagnoses is presented. The frequency of the most common histopathological types was 33% interstitial (Leydig), 26.4% Sertoli and 33.9% seminomas/germ cell tumours. The average age at diagnosis was 10 years, but was significantly lower for Sertoli cell tumours (8.6 years) than for the other tumour types. Following a histopathological re-evaluation, 22.5% of the original tumor diagnoses were modified. Proportional morbidity ratios were calculated and individuals from the breeds Shetland sheepdog and Collie were five times more likely to have testicular tumours than the overall average for the registry. Breed differences in the distribution of histopathologic types were observed. Shetland sheepdog and Collie were most commonly diagnosed with Sertoli cell tumours, while all tumours from Norwegian elkhound in this material were seminomas.     

桦褐孔菌对肺癌细胞抗增殖和诱导凋亡观察

用MTT法测定桦褐孔菌抗突变活性成分Inotodiol,对人肺癌A549细胞株的增殖抑制作用,20mg.L-1～80mg·L^-1桦褐孔菌的抗突变活性成分Inotodiol对人肺癌A549细胞株有抑制增殖作用,且表现出浓度和时间依赖关系。在倒置显微镜下、HE染色光学显微镜下、AO/EB双染色荧光显微镜下、透射电子显微镜下观察,肿瘤细胞均有体积变小、有凋亡小体。桦褐孔菌的抗突变活性成分Inotodiol可抑制人肺癌A549细胞株的增殖和诱导肿瘤细胞凋亡。